STABILITY EVALUATION OF MIXTURES AMONG PREPARATIONS WITH DIFFERENT BIOLOGICAL EFFECT ON BASIS OF GRAIN YIELD IN SPRING FORAGE PEA

DOI: 10.7904/2068–4738–IV(7)–101

Natalia GEORGIEVA, Ivelina NIKOLOVA, Grozi DELCHEV

 Abstract. During the period 2007–2009 in IFC–Pleven is carried out three–factor field trial with purpose to evaluate the stability of mixtures among preparations with different biological effect on basis of the grain yield in spring forage pea. It is used the split plot method. Factor A includes the three years of the study. Factor B (preparations) includes 8 levels: untreated control, Atonic (growth stimulator), Masterblend (combined leaf fertilizer), Atonic + Masterblend, Confidor (insecticide), Confidor + Atonic, Confidor + Atonic + Masterblend, Confidor + Masterblend. Factor C (stages of treatment) includes 3 levels: budding, budding + flowering, flowering. In result of the held experiment is found that the preparations with different biological effect Atonic, Masterblend and Confidor used alone and in mixtures increase the productivity in the spring forage pea (from 101,9 to 129,0%). With highest stability is distinguished the triple mixture Confidor + Atonic + Masterblend (applied at budding stage), followed by mixtures Confidor + Atonic and Confidor + Masterblend (applied at flowering stage) and at alone use of preparations–the leaf fertilizer Masterblend (applied at flowering stage). Technologically the most valuable variants which match high stability and high productivity in the different years are appeared all of them withtwofold treatment of the studied preparations and their mixtures as it is recommended the use of mixtures Confidor + Masterblend and Confidor + Atonic.

 Keywords: stability, pea, preparation with different biological effect

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EFFECT OF 2, 4-D AND ITS COMBINATION WITH NAA AND IAA ON IN VITRO CALLUS INDUCTION OF A LOCAL VARIETY OF CHICKPEA (CICER ARIETINUM L.)

DOI: 10.7904/2068–4738–IV(7)–95

 Kaberi DUTTA, Md. Raihan ALI, S M mahbubur RAHMAN and Md. Shamim AKHTER

Abstract.Epicotyls, hypocotyls & shoot tip were collected as explants from aseptically grown seedling for callus induction. MS media supplemented with different concentrations of 2,4–D (1.0, 2.0, 3.0, 4.0and 5.0mg.L-1) along with NAA (0.2 mg.L-1 or 0.5 mg.L-1) or IAA (0.5 mg.L-1) were used for efficient callus induction. Whitish, soft and watery calli were observed on the media supplemented with 0.5 mg.L-1NAA + 2,4–D (1.0-5.0 mg L-1) and 0.5 mg.L-1 IAA + 2,4–D (1.0–5.0 mg.L-1). The highest (100%) callusing response was obtained in MS + 2.0 mg.L-1 2,4–D + 0.5 mg.-1 NAA after three weeks of culture. Then subculture was done after four weeks for further growth onto same medium. Callus weight was recorded to find out the increasing potentiality of callus at different concentration of auxins.

Key words:  germination, Callus induction, sterilization, regeneration

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STUDY ON THE RESPONSE OF ALFALFA GERMPLASM FOR RESISTANCE TO ROOT ROT

DOI: 10.7904/2068–4738–IV(7)–90

Il. IVANOVA, E. ZHEKOVA, D. MARINOVA, D. PETKOVA

Abstract. The investigation was conducted at IASS “Obraztsov chiflik”–Rousse during the period 2009–2011. Bulgarian and foreign germplasm was the object of study. The objective of the present investigation was to isolate and identify the causes of root rot in alfalfa and to determine the reaction of local and foreign germplasm to the agent. Isolation of the pathogen was performed on KDA and Chapeknutritive medium. Positive results were reported in all germplasm observed. Attack was the lowest in annual plants.

Keywords: alfalfa /Medicago sativa, root rot /root rott, stability

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REACTION OF PARENTAL LINES OF Ruse 555 MAIZE HYBRID TO THE AGRO–METEOROLOGICAL CONDITIONS OF IASS “OBARZTSOV CHIFLIK”–ROUSSE

DOI: 10.7904/2068–4738–IV(7)–86

 Lyubomir Iliev IVANOV, Nataliya Hristova PETROVSKA

Abstract. Maize is a thermophylic and hydrophilic crop. In the region of IASS “Obraztsov Chiflik”–Rousse temperature conditions are favorable for its cultivation, and precipitation and its distribution during vegetation proved to be a limiting factor. The objective of the study was the contrasting influence of the agro–climatic conditions on the mass of 1000 seeds, hectoliter mass and germination of RL 61/31 and H–108 lines to be determined in relation to their seed production and as donors of valuable genes in breeding. The experiment was conducted at the Experimental field of IASS “Obraztsov Chiflik”–Rousse during the period 2006–2008. It was designed after the Block method, in four replications. The results obtained showed that mass of 1000 seeds, hectoliter mass and germination in both lines were the lowest in the year with significant drought. Germination in both lines was strongly influenced by the agro–climatic conditions.

Keywords: maize, parental components, stress, tolerance

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EFFECT OF INCREASING DOSES OF MINERAL NITROGEN FERTILIZATION ON CHEMICAL COMPOSITION OF LUCERNE (MEDICAGO SATIVA L.) UNDER OPTIMUM WATER SUPPLY AND WATER DEFICIENCY STRESS

DOI: 10.7904/2068–4738–IV(7)–80

Viliana Vasileva

Abstract:The effect of increasing doses of mineral nitrogen fertilization (40, 80, 120 and 160 mg N kg–1 soil)on chemical composition of lucerne (Medicago sativa L.) was studied in pot trial at the Institute of Forage Crops, Pleven, Bulgaria. The plants were grown under optimum water supply (75–80% FC) and 10–days water deficiency stress (37–40% FC) at the stage of budding of lucerne. It was found that with increasing doses of mineral nitrogen fertilization, crude protein content in dry aboveground mass increased from 13 to 19% under the conditions of optimum moisture, and decreased from 6 to 14% under water deficiency stress. Crude fiber content in root mass under water deficiency stress increased most in unprovided and provided plants with low dose of mineral nitrogen. Ca:P ratio in dry aboveground mass remainedalmost unchanged in the conditions of optimal moisture, and increased significantly in theconditions of water–deficiency stress.

Key words:lucerne,mineral nitrogen fertilization, water deficiency stress, chemical composition

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EFFECT OF LIQUID ORGANIC HUMATE FERTILIZER HUMUSTIM ON CHEMICAL COMPOSITION OF SPRING FORAGE PEA

DOI: 10.7904/2068–4738–IV(7)–74

Anna ILIEVA, Viliana VASILEVA

Abstract: Effect of liquid organic humate fertilizer Humustim on chemical composition of aboveground biomass, root biomass and grain of spring forage pea was studied at the Institute of Forage Crops, Pleven, Bulgaria (2002–2004). Presowing treatment of seeds and treatments during vegetation were applied. The treatment with Humustim increased content of crude protein, macro elements and water–soluble carbohydrates in the aboveground biomass of plants. The highest content of crude protein (13.69%) and water–soluble carbohydrates (8.40%) was found for presowing treatment with Humustim at the dose of 1.2 L/t seeds + one treatment during vegetation. Applying of Humustim decreased crude protein content in roots from 5.0% to 17.8%. Treatment with Humustim had no effect on crude protein content in grain and increased water–soluble content (from 3.3% to 10.0%) and Са (from 11.1% to 77.8%). Better growth and development of spring forage pea were observed after treatment with Humustim–plant height and root length increased.

Keywords: spring pea, Humustim, crude protein, macro elements, water soluble carbohydrates

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BIOLOGICAL POTENTIAL AND ADAPTIVE CAPACITY OF VARIETIES BIRDSFOOT TREFOIL IN THE ENVIRONMENT OF TROYAN

DOI: 10.7904/2068–4738–IV(7)–69

Boriana CHURKOVA

Abstract. Under the agro ecological conditions of Troyan varieties Bright, Georgia 1, Nueltin, Witt, Pardee, Roseau, Steadfast, Trevig of birds foot trefoil were examined on following indices: dry mass yield, plant height, and morphological composition of sward. The introduced varieties Nueltin (9.0 t/ha–1), Pardee (18.2 t/ha–1), Roseau (12.8 t/ha–1) had the highest dry mass yield in the first, second and third years of the test period. The highest stem per plant was the second year and varieties Bright (47.9 cm) and Nueltin (47.6 cm) were the highest. The highest average leafiness have varieties in the first year of the test period, when it reached 51.2%. The stems prevalent in grass composition in the second year, and generative organs were low during the three years of study. These average results for the period indicate that varieties Witt and Georgia 1 could be success fully cultivated in the agro ecological conditions of Troyan.

Key words: birds foot trefoil, varieties, dry mass yield, plant height, morphological composition

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INFLUENCE OF SOWING PERIOD ON THE FORAGE PRODUCTIVITY AND WEED INFESTATION OF ANNUAL WINTER CEREAL AND LEGUME CROP

DOI: 10.7904/2068–4738–IV(7)–64

Vladimir LINGORSKI

Abstract.During the 2011–2012 period in the Research Institute of Mountain Stockbreeding and Agriculture (RIMSA) in Troyan (Bulgaria) the effect of sowing timeon somebio–productive indicators (green mass and dry matter yields, weed infestation) ofthe forage from annual winter cereal and legume mixture of winter barley and winter pea was performed. As variants the following sowing periods carried out during 10 days were studied: 1. 20th–30th September (Standard); 2. 1st–10th October; 3. 11th–20th October; 4. 21st–30th October. It was established that the most optimal time for sowing of mixed crop grown under foothill conditions of the Central Balkan Mountains (Troyan region) is the period from 1st to 20th October. The received forage amounted to 2.38 t.ha–1 green mass and 0.59 t.ha–1 dry mass, which exceeded the standard sowing period (20th–30th September) with 25.26% and 59.46%, respectively.Irrespective of the autumn sowing period, weed infestation of the crop of winter cereal–legume mixture was comparatively low, ranging from 0.74 to 4.88%.

Key words: winter crop,cereal and legume mixture, sowing time, forage productivity, weed infestation, foothill region, Central Balkan Mountains.

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SOME GENERALISATIONS OF CONFIDENCE INTERVAL ESTIMATES OBTAINED FROM INTERVAL ARITHMETIC

DOI: 10.7904/2068–4738–IV(7)–59

Simon BROWN, David C SIMCOCK

Abstract. The confidence intervals estimated using simple interval arithmetic can be 40% larger than the correct value. Here we provide a general upper bound on this discrepancy which we use to estimate the correct value from the estimate obtained from interval arithmetic. This approximation can be within 4% of the correct value for the kinetic models we examine.

Keywords: confidence band, enzyme kinetics, interval arithmetic.

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FORAGE PRODUCTIVITY AND WEED INFESTATION OF ANNUAL EARLY–SPRING MIXTURE UNDER DIFFERENT SOWING DATES

DOI: 10.7904/2068–4738–IV(7)–54

Vladimir LINGORSKI

Abstract.During the 2011–2012 periods in the Research Institute of Mountain Stockbreeding and Agriculture (RIMSA) in Troyan (Bulgaria) the effect of sowing timeon somebio–productive indicators (green mass and dry matter yields, weed infestation) ofthe forage from annual early–spring cereal–legume mixture from oats and field pea was performed. As variants the following periods of sowing were studied: 1. 10th–20th March (Standard); 2. 21st–30th March; 3. 1st–10th April; 4. 11th–20th April. It was established that the optimum time for sowing of annual early spring pea and spring oats mixed crop was the period from 10th to 20th March. Then was received most plant production (1.84 t.ha–1 green mass and 0.41 t.ha–1 dry matter), which exceeded the other sowing periods from 7.07% (21st–30th March) to 27.72% (11th–20th April) and from 1.6 to 8.13%, respectively. In the different sowing times the weed infestation of crops varied and was a highest in early spring sowing (10th–20th March)–from 8.40 to 9.26%.

Key words: early–spring crop,cereal and legume mixture, sowing time, forage productivity, weed infestation, foothill region, Central Balkan Mountains.

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