SOME REMARKS ON THE KINETIC OF SUCCESSIVE CHEMICAL REACTIONS

Gyorgy BUDAHAZY, Florin CREŢ, Ciprian RUJESCU

Abstract: Successive chemical reactions, often found in technological practice, in agreement with kinetic features available, can be modelled using mathematical equations or systems of differential equations. This paper presents a generelized differential model, for a given number, finite successive reactions; track parameters are changes in concentrations of compouds that generate chemical reaction.

Keywords: successive chemical reactions, mathematical models

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ETIOLOGICAL STUDY OF BLOSSOM BLAST OF PEAR IN SOUTHEAST SERBIA (REGION OF LESKOVAC)

G. JOVANOVIĆ, G. ALEKSIĆ, J. MAKSIMOVIĆ, V. GAVRILOVIĆ, O. ONĆ-JOVANOVIĆ

Abstract: The paper studied and biochemical characteristics of pathogenic strains of bacteria isolated from necrotic pear flower buds in the region of Leskovac. Strains were gram negative, fluoresce on Kings medium B (KB), does not produce oxidase, produce levan and HR caused by tobacco. On the basis of pathogenicity tests, biochemical and physiological properties and molecular methods (PCR) revealed that these symptoms cause the Krušce Psudomnoas syringae, more widespread and economically štetninji pear pathogen not only in Leskovac, but throughout Serbia.

Key words:  Pear, Pseudomnas syringae, blossom blast, Leskovac

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ANCHORAGE INDEPENDENT GROWTH OF NORMAL HUMAN ADULT CHEEK CELLS

Prashant SHUKLA, Jini SHUKLA, Ajay SINGH

Abstract.In vitro growth of adult human cheek cells was obtained in a very simple medium. The medium did not require any growth hormone or serum for the growth of cells. The work is focused on development of a general purpose medium which will facilitate growth of all types of cells in culture without the requirement of either hormones or serum and the pattern of growth obtained in the medium. The growth of human cheek cells in the medium was anchorage independent as a mass of cells.

Keywords: cheek cells, in vitro culture, culture medium

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FATTY ACIDS COMPOSITION AND QUALITY ASSURANCE OF SEMAL (BOMBAX) AND MONSA (CHORISIA) SEED OILS AND USE IN DEEP-FAT FRYING

M. Arafat SHAKER; M. Abd El-Kader ESSAM and R. M. M. SAYED

Abstract: This study evaluates the proximate composition of semal (Bombax) and monsa (Chorisia) seeds, the physico-chemical properties, and fatty acids composition of the seeds oil compared with cotton seed oil. Protein and fat content of semal and monsa seeds were (21.30% and 23.50%) and (28.50 % and 25.15 %), respectively. The major fatty acids components were linoleic (C18:2), Oleic (C18:1) and palmitic (C16:0) in semal and monsa seed oils compared with cotton seed oil. Semal, monsa and cotton seed oils were continuously fried at 180˚C ± 5˚C for 20 hr, 4 hr heating cycle per day for five consecutive days. Aliquots of potato chips were fried in the aforementioned oil samples. Quality assurance testes were performed on non-fried and fried of the oil samples. In generals, the results suggest that semal and monsa seed oil alone and in mixtures with other oil have to ban its use in frying process.

Key words: bombax seed oil, linoleic acid, chorisia seed oil, frying process. 

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INVESTIGATION AND CHARACTERIZATION OF THE GROWTH HORMONE TYPE 1 (GH1) GENE HOMOLOGUE IN THE SALMO TRUTTA CASPIUS

Abolhasan REZAEI, Sheyda AKHSHABI and Hamid Reza JAMALZADEH

Abstract: Salmo trutta caspius growth hormone genes (GH) homologue are used as models for studies in comparative variation breeding, transgenic and growth body weight. Hence variation genetic was studied between the salmo trutta caspius growth hormone gene1 (GH1) and Salmo salar. In this study, isolation and characterization of growth hormone of gene type 1 (GH1) in the salmo trutta caspius, recognizing full length DNA GH1 in strain salmo trutta caspius on the gel electrophoresis. first, DNA genomic extracted from blood samples of adult salmo trutta caspius andfrom places north of Iran was done. The method of DNA extraction (absorption on the fiber glass (kit) were used. One pair of primers was designed towards GH1 gene homologue. DNA template concentration, annealing temperature and extension time were monitored to optimize them. PCR products have been compared between strains on the gel electrophoresis. Four fragments from nucleotide of 1 to 2541 were sequenced. The PCR products were analyzed by BLASTn. Results are shown; there were high homology within strains, salmo trutta caspius and salmo salar 96% approximately. Moreover, our discussion are shown about sequencing homologue GH1 of salmo trutta caspius populations possible have been common ancestor with different species salmo trutta.

Key words:Salmo salar, Salmo trutta caspius,Growth hormone gene type 1, Sequencing, DNA

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STUDIES ON THE POSSIBILITY OF RECOVERY OF SECONDARY PRODUCTS RESULTING FROM THE PROCESS OF VINIFICATION

Alina BUŢU, Marian BUŢU,Steliana RODINO

Abstract. The problem of valorification of wine products was treated differently over time. In the past it was regarded as a minor issue, but today occupies an important place both in researches issues, as well as in economics, because of the global crisis of raw materials and energy, and because of environmental issues involved, too. Under these conditions, research in biotechnology that brings new solutions and can contribute in solving any of these problems is of particular interest. This task is required therefore as a necessity of the economy, with the intention to bring into attention subproducts of a definite value that have a special attention of the specialists abroad, but who are considered waste in our country and treated as such. In this work are presented and analyzed the recovery directions in this field worldwide, as well as research progress in our country.

Keywords:vinification, secondary products, wine yeast, marc

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COLLAGEN USE IN BIOCOMPATIBILITY ENHANCE OF POLYETHYLENE GLYCOL

Elena UTOIU, Maria Lungu, Viorica COROIU, Anca oANCEA

Abstract: New polymeric materials were prepared by mixing a biodegradable, water soluble synthetic polymer (polyethylene glycol–PEG) with a natural polymer with high degree of biocompatibility (type I collagen from bovine tendon). The blends were processed in the form of membranes by mixing aqueous solutions of components and drying of these mixtures. In vitro biocompatibility of polymer mixtures was evaluated by direct contact method, both qualitative methods (cytochemical staining cells with Giemsa) and quantitative methods (determination of cell viability in a culture of human dermal fibroblasts by MTT assay). Evaluation of these materials interaction with cell culture was also made by analyzing the activity of matrix metalloproteinases (MMP) by zymography. It was found that the mixture variant with the best degree of biocompatibility was PEG: Col 1:1 in combination ratio.

Keywords: synthetic polymers, natural polymers, mixtures, polyethylene glycol, collagen, NCTC cell line, biocompatibility, cell viability, matrix metalloproteinase.

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PRODUCTIVITY OF VELVETLEAF (ABUTILON THEOPHRASTI MEDIK.) DEPEND ON ITS DENSITY IN CORN

Eleonora ONC-JOVANOVIC,  Dragana BOZIC, Sava VRBNICANIN

Abstract. Field experiments were conducted in 2006 and 2008 at experimental field in Padinska Skela near Belgrade to quantify productivity of velvetleaf (Abutilon theophrasti Medik.) in corn. The density of velvetleaf artificially created and it ranged from 1 to 8 plants m-1 of corn row. Productivity of velvetleaf were estimate based on plant aboveground biomass and parameters of capsule (number of capsules m-2, number of capsules plant-1, diameter of capsule, number of locules capsule-1) and seed (seed weight m-2, seed weight plant-1, weight of 100 seeds) production. Differences between years in plant aboveground biomass of velvetleafat the stage of inflorescence, as well as in some capsule and seed production parameters were very prominent. Generally, velvetleaf productivity in corn depends of its density, that is with increasing density of population decreases yield.

Keywords: aboveground biomass, capsule production, corn, seed production, velvetleaf.

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ANTIOXIDANT ACTIVITIES OF DATE PITS IN A MODEL MEAT SYSTEM

Amany M. M. BASUNY, M. Arafat SHAKER, Abeer A. KINAWY

Abstract. The aim of the current investigation was to investigate the effect of date pits phenolic compounds compared to BHT as synthetic antioxidant on lipid oxidation and quality of ground beef during refrigerated storage at 0.00±0.50°C for up to 10 days. Khalas variety of date pits (Phoenix dactylifera L.)phenolic compounds was extracted with 4 solvents (Water, methanol, methanol: water (50:50 v/v) and methanol: water: acetone: formic acid (20:40:40:1). Ferric reducing antioxidant power assay (FRAP) and Folin–Ciocalteu reagent was used for determination of the antioxidant activity and phenolic content of date pits. Results indicated that the highest antioxidant was shown by the date pits extract (Water: methanol: acetone: formic acid), therefore 0.5, 0.75 and 1.00 % of either date pits extract and BHT were added to minced meat and evaluate it’s effects on the lipid peroxidation of ground beef during storage process. TBA test as quality assurance test was conducted at the beginning of the experiment and after 2, 4, 6, 8 and 10 days of storage. The results of this study showed that the date pits extract (Water: methanol: acetone: formic acid) had significantly the highest levels of total polyphenols and antioxidant activity. Also, the obtained results indicated that phenolic compounds in date pits of khalas variety had high antioxidative effect in reducing the formation of hydroperoxides during storage.

Key words: phenolic compounds, antoxidative activity, date pits, FRAP method, fatty food.

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STUDY ON CICADA AND TRIPS ENTOMOFAUNA IN WINTER VETCH (VICIA VILLOSA ROTH.)

I. NIKOLOVA, N. GEORGIEVA

Abstract. In 2007-2010 period was studiedthe quantitativeand qualitative composition of sucking pests from HomopteraAuchenorrhynchaandThysanopterainwinter vetch agrocenosis in the conditions of Pleven region as well as the numerical dynamics of the most economicallyimportantspecies of them. For Pleven region in winter vetch from Homopteraorder, Auchenorrhynchawere established cicadas belonging to 5 families, 14 genera и 14 species. Economic importance for winter vetch had Empoasca pteridiswhich was found from the beginning of May to the harvesting of culture as its density increased in the reproductive stages of vetch flowering and beginning seed fillwith maximum in the beginning of July. FromThysanopteraorder was foundtripswhichbelonged to 3 families with 6 species. Asimportantpestswere outlined Thrips tabaci (53.6%), Taeniothrips atratus (15.6%) andOdontothrips confusus (15.0%).The density of Thrips tabaci increased in May and reached the highest values in the first decade of June as Taeniothrips atratus andOdontothrips confususwerein higher density from the second half of May to the middle of June. To useful species relatedAeolothrips intermedius which because of its high consumptive ability of predatory might control the density of harmful trips in determinate numbers and to contribute for decrease of their density.

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