Raed Kadhim JASIM
The Iraqi Ministry of Education, Educational Directorate of Basra, Alackramen Preparatory school for boys, Basra, Alackramen, IRAQI
*Corresponding author: Mobile: +9647702254154, Email: email@example.com
Abstract. The purpose of this study was to determine the effect of some cultural conditions on the Xylanase enzyme production by Sporolactobacilli isolated from the industrial soil and to investigate their potential to produce xylanase utilizing grass and other materials as a substrate. Xylanase activity was detected using the Dinitrosalicylic acid assay method. It was isolated from the industrial soil, secrets extra cellular xylanases when grown in liquid media supplemented with rice bran, grass, corncob, or sugar baggage as a carbon sources (which were treated with 2N NaOH for removing the cellulose from these substrates). The maximal enzyme production was obtained when rice bran xylan was used as a carbon source. It was shows the high enzyme activity at high temperatures 55ºC and high enzyme activity was found at pH 8. The extra cellular enzyme has an apparent molecular weight of 68 KD, as determined by SDS–PAGE. The purified enzyme was confirmed by Zymogram analysis. The Sporolactobacilli show high enzyme activity of 4.7 U/mL. The newly isolated bacterial species in this experiment are having alkalophilic and thermophilic enzymatic properties, which is advantageous to maintain the conditions during industrial processing.
Keyword: Sporolactobacilli, industrial soil, xylanase.