Molecular Characterization of Hyalomma dromedarii from Bikaner, India

HARISH K. CHANGAL1, G. NAGARAJAN2*, R. K. PUROHIT1, SHELESH KUMAR SWAMI2, S.C. MEHTA2 and K.M.L. PATHAK3

DOI: 10.7904/2068–4738–V(10)–52

 1Radiation Biology Laboratory, P.G. Department of Zoology, Govt. Dungar College, Bikaner, Rajasthan, INDIA. 2National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner–334 001, Rajasthan, INDIA. 3Indian Council of Agricultural Research, Krishi Bhavan, New Delhi–110001, INDIA.*Corresponding author: Dr. G. Nagarajan, M. V. Sc., Ph.D., Senior Scientist, Southern Regional Research Centre under Central Sheep and Wool Research Institute, (CSWRI), Mannavanur, Kodaikanal, Tamil Nadu–624 103, INDIA; e–mail: camelnag@yahoo.com; gnagarajanars@gmail.com. Phone: +91–4542–276414; Fax: +91–4542–276413

Abstract. In this study, partial nucleotide sequences of cytochrome oxidase (COXI) gene and P–18 protein gene of Hyalomma dromedarii from one humped camels of National Research centre on Camel, Bikaner, India, were amplified by polymerase chain reaction from the salivary glands of the ticks. Sequence analysis revealed that COXI gene of H. dromedarii from Bikaner shared 99.1–99.2 % sequence identity at the nucleotide level with H .dromedarii isolates from Kenya and Ethiopia, respectively. With other species of Hyalomma, H. dromedarii from India exhibited 86.2–90.2 nucleotide identity. With other genera of the family Ixodidae, Hv. dromedarii from India showed 82.9–84.78 % nucleotide identity. But, salivary gland protein gene P–18 of H. dromedarii from Bikaner was having 90.4 % sequence identity with that of H. asiaticum.

Key words: Hyalomma dromedarii; Dromedary camel; National Research centre on Camel; Bikaner; India

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