Surya Prakash D.V.1, Meena V.2
1Centre for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam–530003, Andhra Pradesh, INDIA. e–mail: firstname.lastname@example.org. 2Centre for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam–530003, Andhra Pradesh, INDIA. e–mail: email@example.com
Abstract. Chebulinic acid is a phenolic compound found in the fruits of Terminalia chebula (Haritaki), Phyllanthus emblica (Amla) and seeds of Dimocarpus longan (Longan) species etc. It showed many pharmacological activities including inhibition of cancer cell growth like human leukemia K562 cells, colon adenocarcinoma HT–29 cell lines, anti–neisseria gonorrhoeae activity etc. The present studies on optimization of physico–chemical parameters like effects of different solvents, soaking time, extraction time with hexane, particle size, different solvent percentages, different volumes of hexane with ethanol and methanol as solvents, pH and different weight dosages for the extraction of Chebulinic acid from Medicinal herbs. The highest chebulinic acid concentration for optimized conditions of Terminalia chebula fruit, Amla fruit and Longan seeds were 6.2 mg/mL, 4.8 mg/mL and 5.7 mg/mL respectively.
Key words: Chebulinic acid, Terminalia chebula fruit, Amla fruit, Longan seed, Folin–Denis reagent