TISSUE CULTURE OF Simmondsia chinensis (Link) SCHNEIDER

DOI: 10.7904/2068–4738–VI(11)–45

Ahmed M. EED*, Adam H. BURGOYNE

Department of Plant Production. College of Agriculture and Veterinary. Ibb University. YEMEN.
(e–mail: am_eaid@yahoo.com, M: 00967–772876682)
Jojoba Naturals Corporation, Montevideo, URUGUAY.

Abstract. Jojoba (Simmondsia chinensis (Link) Schneider) a multipurpose and monogeneric dioecious shrub from arid zones, has emerged as a cash crop all over the globe. Its seed propagation poses severe problems due to its male–biased population: the male: female ratio is five to one. To overcome this phenomenon, asexual propagation using vegetative methods such as tissue culture could be used. Investigations were conducted for propagating jojoba plants aseptically in vitro staring from nodal segment with two full or half leaves and shoot tip explants. The growth media (MS) were a basal medium additional to Gamborg’s vitamins (B5) and then modified MS medium (MMS) supplemented with different plant growth regulators (PGRs). The results indicated that MS–B5 growth medium was better than MMS medium after 40 and 60 days of culture. Shoot tip explants were superior to nodal segments with two full or half leaves at different concentrations and combinations of PGRs. The highest rooting percentage was recorded in MS/2–B5 + 1 mg/L IBA. This procedure produced an efficient protocol for jojoba tissue culture.

Keyword: Explant, GA3, In vitro, Jojoba, PGRs

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