EVALUATION OF EXON 17 OF INSULIN RECEPTOR (INSR) GENE AND ITS RELATIONSHIP WITH DIABETES TYPE 2 IN AN IRANIAN POPULATION

 DOI: 10.7904/2068–4738–VII(13)–61

Abolhasan REZAEI¹*, Sheyda AKHSHABI¹, Fariba SADEGHI2

 1Department of Genetics–School of Basic Science, Islamic Azad University, Tonekabon Branch, IRAN

2Department of Medicine, Islamic Azad University, Tonekabon Branch, IRAN

 Corresponding Author: Abolhasan Rezaei, e– mail: a.rezaei@tonekaboniau.ac.ir

 Abstract. Mutations in insulin receptor gene cause the inherited insulin resistant syndrome, especially diabetic diseases. Here were optimized the conditions for sequencing of a partial fragment of INSR gene, exon 17. We sequenced fourteen fragments from a diabetic sample and a control group chosen from an Iranian population. In this study we analyzed eleven sequences of diabetic’s patients by DNAMAN program (DEMO 8.0), deposited in Genbank with accession numbers LC055416, LC055417, LC055419, LC055421, LC055423, LC055424, LC055425, LC055426, LC055496, LC055497, and LC055498. From our control group, three sequences were deposited in Genbank with accession numbers of LC055418, LC055420, and LC055422. Results showed that there were variation between the sequences of exon 17 of INSR gene in diabetics and control group. Variations were observed in fragments at the beginning and the end of exon 17 among diabetics population. Moreover we found subjected SNPs between diabetic’s patients that haven’t been reported by other researchers. In this study we concluded that mutations in exon 17 of INSR gene contributed in diabetic diseases.

Keyword: Insulin receptor gene, Exon 17, Diabetes, Sequencing, Control group

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