Nawel OUIS1*, Ahmed HARIRI2
1Laboratory of Physical Chemistry of Macromolecules and Biological Interfaces, Faculty of Science the Nature and Life, University Mustapha Stambouli of Mascara (UN 2901), BP. 763, Sidi Said, 29000, Mascara, ALGERIA
2Bioconversion Laboratory, Microbiology Engineering and Health Safety, Faculty of Science the Nature and Life, University Mustapha Stambouli of Mascara (UN 2901), BP. 763, Sidi Said, 29000, Mascara, ALGERIA
*Corresponding author: email@example.com
Abstract. The present study investigates the antioxidant and antibacterial activities of the essential oils obtained by hydrodistillation of the pulp and the seeds of Ceratonia siliqua. Dried pods (pulp or seeds) were subject to the hydrodistillation for 3 h using a Clevenger–type apparatus. The two essential oils obtained were characterized by its physicochemical indications. Theses oils were used for the determination of the antioxidant activity by free radical scavenging activity, reducing power and liver lipid peroxidation methods and for the evaluation of the antibacterial properties against referenced tested bacteria. The oil of the pulp presents a high scavenging power of free DPPH radical 89.0 ± 1.2 % at concentration 1000 µg/mL and IC50 7.8 µg/mL compared to the seeds 79.0 ± 1.6 %, IC50 31.25 µg/mL. A significant activity for reducing iron and protection against lipid peroxidation induced by Fe+2 were obtained by the oil of the pulp. The essential oils of carob showed a good antibacterial activity against tested referenced pathogenic bacteria Staphylococcus aureus ATCC 25923, Pseudomonas aerugenosa ATCC 25853 and Escerichia coli ATCC 25922.
Keyword: Antibacterial, Antioxidant, Carob, Essential oils, Pulp, Seeds.