In vitro mutagenic effect of cedar (Cedrus libani A. Rich) tar in the salmonella/microsome assay system

DOI: 10.7904/2068-4738-X(20)-13

Hatice Aysun MERCIMEK TAKCI1*, Filiz Ucan TURKMEN2, Mehmet SARI3

 1Kilis 7 Aralık University, Faculty of Arts and Sciences, Molecular Biology and Genetics Department, 79000 Kilis, TURKEY

2Kilis 7 Aralık University, Faculty of Engineering and Architecture, Food Engineering Department, 79000 Kilis, TURKEY;

3Mersin University, Medical Faculty, Basic Medical Sciences, Mersin, TURKEY

Corresponding author: mersimek@hotmail.com

 

Abstract. Cedar tar is obtained by pyrolytic breakdown of Cedrus libani A. Rich (Pinaceae) wood. Cedrus libani A. Rich (Pinaceae) grows on the Taurus Mountains in Southern Turkey, Eastern Mediterranean, Western Syria and Lebanon. Objective: The main objective of this study is to investigate the mutagenic and antioxidant effect of Cedar tar produced from cedarwoods collected from Gume village of Mut, Mersin, Turkey. Methods: The mutagenic activity of Cedar tar was screened by using Salmonella typhimurium TA98 and TA100 strains, with and without S9 metabolic activation in this study. Three concentrations of (10, 15 and 25 mg/plate) Cedar tar were examined in AMES assays. Results: Cedar tar was found to be non–mutagenic against strains TA98 and TA100 (p˃0.05) in the presence and absence of S9 metabolic activation. Cedar tar did not show any antioxidant activity based on DDPH radical scavenging. The total phenolic and flavonoid contents of tar were 0.85±0.06 mg GAE/g and 0.068±0.02 mg RE/g, respectively. Conclusions: Antimutagenic, cancerogenic and cytotoxic effects of Cedar tar should also be investigated by using different test systems.

Keyword: Ames test, Cedar tar, Folk medicine, Mutagenicity.


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