Kim–Bup NGUYEN1, Gia–Buu TRAN2, Hong–Thien VAN2*
1Dong Thap University, 783 Pham Huu Lau Street, Cao Lanh City, Dong Thap Province, VIETNAM
2Institute of Biotechnology and Food–technology, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap District, Ho Chi Minh City, VIETNAM
*Corresponding author: firstname.lastname@example.org
Abstract. Wax apple (Syzygium samarangense) is a popular species with high economic value in Vietnam and other countries in Asia. This research analyzed and compared morphological and molecular characteristics (ITS and trnL–F sequences) of 5 varieties of wax apple in Dong Thap Province, Vietnam, including of An Phuoc, Xanh Duong, Hong Dao, Hoa An, and Sua wax apples. The results revealed that five varieties of wax apples had a board range of morphological characteristics, especially in shape, color, and size of fruits. Furthermore, molecular analysis results showed that ITS and trnL–F sequences of four varieties of wax apples, including of Xanh Duong, Hong Dao, Hoa An, and Sua, were identical with 100 % similarity whereas An Phuoc wax apple had differences in one and five positions in ITS and trnL–F sequences as compared to other varieties. The phylogenetic tree was established by comparison of ITS sequences of five varieties in this study and other varieties in Genbank (NCBI) and showed that four varieties of wax apples (Xanh Duong, Hong Dao, Hoa An, and Sua) were 100 % match with two varieties grown in China (KC815987, KC800610) and one variety planted in Sri Lanka (MN104146). On the other hand, ITS sequence of An Phuoc wax apple was high similarity with another wax apple variety planted in Sri Lanka (MN104142).
Keyword: Wax apple, ITS, trnL–F, Phylogeny.