Optimization of extraction technology for determination of caffeic and chlorogenic acid in dandelion

DOI: 10.7904/2068–4738–XI(21)–26

 

Zhe WU, Zhaojia LI, Zhizhong XUE, Xuelin LU, Xiuping WANG*

 

Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, 63 Binhai street, Caofeidian, Tangshan 063299, CHINA

*Corresponding author: wuzhe26@163.com Tel: +86 315 8723260

 

Abstract. In order to obtain the optimum extraction technology for rapid and accurate determination of phenolic acids from dandelion in laboratory, methanol was used as extractant, and response surface methodology (RSM) was used to optimize the extraction process combined with the quality evaluation by the fingerprint analysis. Results showed that the input of cellulase was beneficial to the stability of the extraction. The liquid–material ratio promoted the extraction rate, and the methanol with the extraction time or the liquid–material ratio showed synergistic effect on the increase of extraction rate. Chlorogenic acid and caffeic acid were the main compounds and their match degree with the control fingerprint map were all 100 % according to the HPLC fingerprint analysis. Thus, the two compounds were used as main evaluation indexes to obtain the optimal extraction conditions, namely that liquid–material ratio of 300, methanol concentration of 40% and ultrasonic extraction time of 120 min. The obtained extraction process reduced the operation cost and maintained the stability of detection results. It could be directly used for the determination of phenolic acids from dandelion in laboratory.

Key words: caffeic acid, chlorogenic acid, dandelion, fingerprint, optimization.

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