Mahdieh GHOLIPOUR1, Bahram BAGHBAN KOHNEHROUZ1*
1Department of Plant Breeding and Biotechnology, Faculty of Agriculture University of Tabriz, Tabriz, IRAN
*Corresponding author: firstname.lastname@example.org, Tel: +98 41 33392031, Fax: +98 41 33356003
Abstract. One of the advantages of chloroplast engineering is the entrance of targeted gene in to a specific region of the chloroplast genome. Due to advantage of this method than nuclear genetic engineering, it is one of the safest ways for the production of recombinant bio-pharmaceuticals, vaccines In this research, cloning of ndhB–rps7 fragment was considered from lettuce chloroplast genome as flanking regions. The designing of specific primers for amplification of these regions was done by Primer 3 and Oligo Analyzer softwares. After total genomic DNA extraction, PCR amplification of fragment was done and cloning of this fragment was This work is the first step in the production of biomaterials in plants.
Keyword: Plastid Genome, flanking region, ndhB–rps7, Homologous recombination