Adaptability and Stability of White Lupin Cultivars

DOI: 10.7904/2068–4738–IX(18)–65

Natalia GEORGIEVA1*, Valentin KOSEV2

 1Department of Technology and Ecology of Forage Crops, Institute of Forage Crops, Pleven, Bulgaria

2Department of biochemistry, Institute of Forage Crops, Pleven, Bulgaria

Corresponding author:, Phone: +35964805882

Abstract. Need to create cultivars combining high potential productivity and resistance to stressful environmental factors appears a new priority in the plant breeding. The present study aimed to estimate adaptability and stability of eleven white lupin cultivars regarding seed productivity and main yield components. The experiment was conducted during 2014–2016 at the Institute of Forage Crops (Pleven), by using randomized block design. The obtained results showed a significant genotype–environment interaction for all studied traits (with exception of pod length and pod width). The calculated parameters determined environmental stability in regard to the main traits in genotypes as it follows: for the plant height–Amiga and Kijewskij Mutant, for number of pods per plant–Nahrquell and Amiga, for seeds per plant–Ascar and Amiga, for the first pod height–Kijewskij Mutant and BGR 6305, for seed weight per plant–BGR 6305 and Garant, for 1000 seed weight–Shienfield Gard and Garant. Cultivar Hetman was stable, but had low values of the studied traits and exhibited low adaptive ability (bi<1). Based on the conducted study, cultivars BGR 6305, Amiga and Garant could be used as source material in lupin breeding program for development and selection of stable and high–productive lines.

Keyword: adaptability, cultivars, environment, stability, white lupin.


Evaluation of Romanian alfalfa varieties under the agro–environmental conditions in northern Bulgaria

DOI: 10.7904/2068–4738–IX(18)–56

Diana H. MARINOVA, Iliana I. IVANOVA, Evgeniya D. ZHEKOVA

 1Institute of Agriculture and Seed Science “Obraztsov chiflic”, 1 “Prof. Ivan Ivanov” str. 7007 Rousse, BULGARIA,

*Corresponding author:

Abstract. The aim of the present study was to determine forage productivity, crude protein content, grass stand height and regrowth rate of Romanian alfalfa varieties in the specific agro–environmental conditions in Northern Bulgaria during four growing seasons. Six Romanian alfalfa varieties–Catinca, Magnat, Madalina, Sandra, Roxana and Daniela were included in the experiment. Bulgarian Prista 3 variety was used as a standard. The analysis of results for studied traits showed that with good performance concerning both forage productivity and rapid recovery after cutting Sandra variety was distinguished. Regarding dry matter yield and crude protein content, for four years of study no significant differences were detected among the alfalfa varieties. The yields of Romanian varieties were satisfactory and less below (2.22 %) the average yield produced by Prista 3 local variety. With respect to the crude protein (CP) content, as the most important quality parameter, the studied varieties fall into the medium CP content group, excepting Prista 3 and Roxana varieties falling into the high CP content group. There were found statistically significant differences among the varieties regarding grass stand height and regrowth rate after cutting. The studied Romanian varieties are characterized by high forage productivity and high crude protein content in dry matter. They could be evaluated as the varieties suitable for cultivation in the conditions of the Northern Bulgaria.

Keyword: alfalfa, varieties, crude protein, dry matter yield, grass stand height, regrowth.

Utilization of locally available binders for densification of rice husk for biofuel production

DOI: 10.7904/2068–4738–IX(18)–47

Mohammed BAKARI1*, Hajara Oyiza YUSUF2

 1Department of Bioresource Engineering, Macdonald Campus, McGill University, 21.111 Lakeshore Road, Ste–Anne–de–Bellevue, Quebec, CANADA H9X 3V9; MAUTECH, Yola, Adamawa State, NIGERIA

2National Biotechnology Development Agency, P.M.B. 5118 Wuse Zone 3, Abuja, FCT, NIGERIA

Corresponding author: and

Abstract. Sustainability of rice processing depends to a large extent on the utilization of waste by–products generated during the process as biofuel feedstock, biomaterial feedstock or animal feed. Rice husk is an abundant by–product of rice processing that is dumped within the rice processing communities with negative environmental effects. Densification of rice husk into pellets and briquettes using available binders from trees used as firewood, and other agricultural wastes will make it an effective biofuel feedstock that will increase sustainability of rice production. In this study, three locally available binders (Afzelia africana aril, de–oiled A. africana aril, and groundnut shell) were chemically and physically characterize; and their effectiveness as binders for production of densified rice husk briquettes for biofuel production were studied. The study showed that binder chemical properties affect the permeability and density of the densified rice husk briquettes.

Keyword: rice husk, sustainability, biofuel, densification, Afzelia Africana aril, groundnut shell.

Effect of biological products on the population of aphids and chemical components in alfalfa

DOI: 10.7904/2068–4738–IX(18)–38

Ivelina NIKOLOVA1*, Natalia GEORGIEVA1

 1Department Tehnology and ecology of forage crops, Institute of Forage Crops, Pleven, BULGARIA

Corresponding author: Tel. +359 884684575

Abstract. One of the most serious pests of commercial Fabaceae crops especially alfalfa is the pea aphid Acyrthosiphon pisum Harris. The pea aphid damages crops directly caused chlorosis and necrosis, leading to significant alfalfa loss worldwide. Hence the need for aphid management and use of insecticides. It was studied the effect of a biological insecticide Agricolle and two biological nano–fertilizers (Nagro and Lytovit) to a control of pea aphid, A. pisum (Hemiptera, Sternorrhyncha: Aphididae) and changes in plastid pigment and total nitrogen contents in alfalfa. On the basis of the sweeping with the entomological net average number of aphids and mortality were calculated on the 1, 5, 7 and 9 days after treatment. It was found that the treatment with biological products Agricolle, Nagro and Litovit used alone and in combinations reduced A. pisum density in a different range. After application of Agricolle with Nagro combination, followed by Agricolle was found the lowest density and the highest aphid mortality. Biological products increased the total pigment content as the highest impact demonstrated used of Agricolle with Nagro–33.4 % increase, followed by treatment with Nagro–29.6 %. Chlorophyll a to chlorophyll b as well as green pigments to carotenoids ratio determined plants treated with Agricolle + Nagro and Nagro as plants with the best physiological state. Alfalfa fixed the most nitrogen (respectively more with 2.77 and 2.95 kg N/kg dry mass yield in compared to control) in the combined treatment of Aricolle with Nagro and Litovit.

Keyword: Agricolle, nano–fertilizers, Acyrthosiphon pisum mortality, plastid pigments, nitrogen.

Effect of different levels of Fenugreek powder supplementation on performance, Influenza, Sheep red blood cell, New Castle diseases anti-body titer and intestinal microbial flora on Cobb 500 broiler chicks

DOI: 10.7904/2068–4738–IX(19)–29

Yaser RAHIMIAN1*, Sayed Mostafa AKBARI2, Morteza KARAMI3, Mostafa FAGHANI1

1Department of Animal Sciences, Shahre-Kord Branch, Islamic Azad University, Shahre-Kord, IRAN

2Department of Animal Sciences, Yasooj Branch, Islamic Azad University, Yasooj, IRAN

3Department of Animal Sciences, Agriculture and Natural Resource Research Center, Shahre-Kord, AREEO, IRAN

Corresponding author:

The aim of this study was to investigate the effect of using fenugreek powder on performance, some immune parameters and intestinal micro-flora on broiler chicks. A total of 350 one day Cobb 500 broiler chicks were divided and assigned into 7 groups and 5 replicates of 10 birds each. Chicks were fed by basal diet as control, 3 levels (0.7, 1.4 and 2.8 percentage) of fenugreek powder respectively. During the experimental period feed intake (FI), body weight gains (BW) and feed conversion ratio (FCR) were calculated. Also, after 30 days old blood samples were taken from wing vein to evaluate the sheep red blood cell (SRBC) and anti-body against Influenza and New castle disease vaccine (ND). For evaluation carcass traits 4 birds of the same weight in each group were slaughtered, separated and weighed. The result obtained from this study showed that the highest FI related to the fenugreek powder and highest BW was seen in the groups that the fed by 2.8 % powder. Also, there were significant differences between treatments about FCR (p≤0.05). The SRBC, ND and Influenza titer as an immune system was for 2.8 % fenugreek powder respectively. The carcass evaluation mentioned that the highest carcass percentage was for 2.8 % fenugreek powder and there were significant differences between groups about intestine and gizzard percentage. The results showed that different levels of fenugreek powder used in experimental broilers had significant effects on intestinal microbial population. We also concluded that fenugreek powder at the present levels can enhance body performance, some carcass visceral percentage, some immune system parameters titer in Cobb 500 broiler chicks.

Key words: Broiler, Fenugreek, Performance, Immune system, Intestinal microbial population.

The purification performances of the lagooning process, case of the Beni Chougrane region in Mascara (Algerian N.W.)

DOI: 10.7904/2068–4738–IX(18)–20

Laidia ZERKAOUI1 *, Mohamed BENSLIMANE1, Abderrahmane HAMIMED2

 1Laboratory of Geomatics, Ecology and Environment (LGEE), Faculty of Sciences of Nature and Life, University Mustapha Stambouli–Mascara, ALGERIA, Phone/Fax: (+213) 45 707 019,

2Biological Systems and Geomatics Research Laboratory (LRSBG), Faculty of Natural and Life Sciences, Mustapha Stambouli University of Mascara, ALGERIA

Corresponding author:, Phone: (+213)671132713

Abstract. In order to alleviate the water shortage observed in rural areas, Algeria has resorted to the use of treated wastewater from lagooning stations. The approach to the management of domestic wastewater is all the more interesting because it is part of an environmental context for the collection, treatment and recycling of treated water for agricultural purposes. This is lagooning as a purification process. The question is whether it meets environmental and health requirements. The present work aims to monitor and analyze the purification performance of six sewage treatment plants located in the region of Mascara (West Algeria) namely: Ghriss, Bouhannifia, Hacine, Mohammadia, Tizi and Froha, to examine the reliability of this wastewater treatment system in accordance with universal standards. We notice that purification performances are low and variable and remain dependent on the season for nitrates (NO3), ammonium (NH4+) and phosphorus.

Keyword: performance, clean water, lagooning, Beni Chougrane, Algeria.

Botanical and morphological composition of artificial grassland of bird’s–foot–trefoil (Lotus Corniculatus L.) treated with lumbrical and lumbrex

DOI: 10.7904/2068–4738–IX(18)–12


 1 Research Institute of Mountain Stockbreeding and Agriculture, 5600–Troyan,

281 Vasil Levski Street, Troyan, BULGARIA

Corresponding author: E–mail:

Abstract. In the period of 2014–2016 at the Research Institute of Mountain Stockbreeding and Agriculture–Troyan, Lumbrical and Lumbrex bioproducts were tested in a field experiment on a grassland of bird’s–foot–trefoil with ‘Leo’ cultivar. Organic fertilizers are high in humic and fulvo acids, macro and micro elements. They are a result of the processing of organic waste through the red earthworm (Lumbricus rubellis) and the application of modern biotechnology. The results show that the independent introduction of the studied preparations has a positive influence on the density of Lotus corniculatus L. in the grassland. The main crop in the harvested biomass prevails in the soil and leaf feeding variants with 3.9 and 1.2–2.7 percentage units, respectively. The weed vegetation is reduced more significantly mainly in Lumbrical variants, regardless of the fertilization level. The liquid fraction of Lumbrex, introduced in the bud–formation period – beginning of blossoming at a dose of 150 and 200 mL/da, had a slighter influence on the participation of bird’s–foot–trefoil (89.8–91.4 %) in the total biomass as well as the level of weed infestation, while it increased more significantly the amount of leaf mass. The percentage share of variants with foliar treatment exceeded the control with 7.0 (Lumbrex 150 mL/da) to 10.4 (Lumbrex 200 mL/da), and those with soil nutrition with 1.5 (Lumbrical 150 mL/m2) and 4.3 (Lumbrical 200 mL/m2) percentage units.

Keyword: Lotus corniculatus L., Lumbrical, Lumbrex, bio–fertilization.

Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

DOI: 10.7904/2068–4738–IX(18)–5

Mohammad MAHMOODI1, Kian Pahlevan AFSHARI1*, Hamid Reza SEYEDABADI2, Mehran ABOOZARI1

 1Department of Animal Science, Faculty of Agriculture, Abhar Branch, Islamic Azad University, Abhar, IRAN

2Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IRAN

Corresponding author:

Abstract. This study was to determine phylogenetic relationships and genetic variation in Iranian Afshari sheep breed. For this reason, phylogenetic relationships and genetic variation were analyzed by using 12S rRNA and 16S rRNA gene sequences. The genomic DNA was isolated by salting out method and amplified 12S rRNA and 16S rRNA genes using PCR method. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 859 and 1053 bp lengths, respectively. Sequence analysis was performed using Bio–Edit software. Phylogenetic tree was constructed using MEGA software. Phylogenetic analysis of haplotype in the combination with the sheep from Gen–Bank showed that Iranian Afshari sheep made a close to the Australian sheep cluster. There was found informative for establishing relationships between breeds from different parts of the world. This may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.

Keyword: 12S rRNA, 16S rRNA, Phylogenetic analysis, Iranian Afshari sheep.