Uche Ndubuisi EGU1 Josiah Chidiebere OKONKWO2
1Department of Animal Science and Fisheries, Abia State University, PMB 7010, Umuahia, NIGERIA
2Department of Animal Science and Technology, Nnamdi Azikiwe University, PMB 5025 Akwa, NIGERIA
Corresponding author: firstname.lastname@example.org, email@example.com
Abstract. Twelve sexually matured (2–2.6 years old) healthy Balami rams were used to determine the effect of Gonadotrophin (Diclair®) on semen characteristics, body conformation and hormonal profile. The rams were divided into 3 treatment groups of 4 rams, identified as T1 (control), administered with 1.00 mL physiological saline, T2, administered with 54.00 i.u Diclair® (0.36 mL), T3, administered with 108.00i.u Diclair® (0.72 mL) with one ram per replicate in a Completely Randomized Design (CRD). The injections were divided into three doses each and administered intramuscularly in the thigh for three consecutive days. The results showed that there were significant differences (P < 0.05) among the treatment groups in all the parameters for semen quality: semen volume, individual motility, sperm concentration, proportions of live, normal and dead sperm cells except semen pH which was similar (P > 0.05) among the treatment groups. The results further showed that there were significant differences (P < 0.05) among the treatment groups in luteinizing hormone (LH) and testosterone levels in the serum. However, there were no significant differences (P > 0.05) among the treatment groups follicle stimulating hormone (FSH) level in the serum. Similarly, the results showed that there were significant differences (P < 0.05) among the treatment groups in urea, glycerophosphocholine, ascorbic acid, sodium, potassium, bicarbonate and fructose levels in the seminal plasma. The results of this study showed that Diclair® enhanced semen quality and was not detrimental to the hormonal profile and biochemical constituents of the seminal plasma of the Balami rams.
Keyword: Diclair®, semen quality, hormones, seminal plasma constituents, Balami rams.