Constantin DANIEL, Liana TOMA

Abstract. Glioblastoma is the most common malignant neoplasm that occurs in the central nervous system in humans, it is very difficult to treat despite advanced diagnostic, imaging and surgical techniques. In recent decades, great efforts have been made to elucidate the cellular and molecular mechanisms of this disease. However, not much progress has been made due to the fact that the brain tumor is composed of heterogeneous populations of cells, many of them resistant to chemotherapy. It is clear that researchers need many in vitro test models (cell lines) in order to better understand and treat this disease. The purpose of this study is to compare in terms of the amount of DNA and cell cycle, a malignant glioma cell line, T11 obtained at INCDSB 1, with a stabilized glioblastoma cell line acquired from a reference center (U87 line). The flow cytometry analysis determines whether the cell cycle and DNA content of our local cell line, T11, are similar to those of the U87 line used worldwide.Our results indicate that are no significant differences between the two cell line regarding size, granularity, DNA content and cell cycle.

Keywords: glioblastoma, cell cycle, flow cytometry.